Elsevier

Clinical Biochemistry

Volume 43, Issue 6, April 2010, Pages 566-570
Clinical Biochemistry

Decrease in antioxidant status of plasma and erythrocytes from patients with ankylosing spondylitis

https://doi.org/10.1016/j.clinbiochem.2009.12.019Get rights and content

Abstract

Objectives

The aim of the study was to evaluate the antioxidant status in plasma and erythrocytes from patients with ankylosing spondylitis (AS).

Methods

16 patients with AS and 16 healthy volunteers were involved in this study. Activities of antioxidant enzymes: superoxide dismutase (SOD) and its isoenzymes — (SOD-Mn) and (SOD-ZnCu), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione transferase (GST), as well as the total antioxidant status (TAS) and concentration of malondialdehyde (MDA) in plasma and/or erythrocytes, respectively were determined.

Results

In patients with AS, a statistically significant decrease in plasma activity of SOD, SOD-CuZn and TAS, significant drop in activity of SOD, GPx, GST and GR in erythrocytes, as well as increased concentration of MDA in comparison with control group of healthy volunteers was observed.

Conclusion

Decrease in antioxidant status leading to generation of oxidative stress may play an important role in the pathogenesis of ankylosing spondylitis.

Introduction

Ankylosing spondylitis (AS) is a chronic inflammatory disease of the locomotor system, which may contain periods of aggravation as well as remission, or be chronically progressing, leading to reduction of mobility of the spine and chest. AS most often begins with inflammation of the sacroiliac joints. As the disease progresses, the inflammatory process comprises apophysial joints of the spine, costal cartilages, as well as tissues and ligaments of the spine, leading to their gradual ankylosis. Of the peripheral joints, the most often affected ones include hip, knee, or talar joints. The disease concerns mainly men, its first symptoms occur at the age of 20–30 years [1], [2], [3], [4].

The pathogenesis of AS is yet still unknown. Most authors suggest autoimmune and genetic mechanism, role for several proinflammatory cytokines including interleukines 23 and 17, but also reactive oxygen species (ROS) might be involved in this process [2], [3], [5], [6], [7], [8], [9], [10], [11], [12], [13].

At the present stage of knowledge it is known that ROS take part in the pathogenesis of many pathological processes and diseases. Their participation has been confirmed, among others, in the pathogenesis of inflammatory conditions, ageing process, atherosclerosis, myocardial infarction, Alzheimer's disease, diabetes, neoplastic diseases, as well as various diseases of the locomotor system [14], [15], [16].

Taking into account data presented above the aim of the study was to assess the oxidant/antioxidant status in patients with ankylosing spondylitis in comparison to healthy persons.

Section snippets

Patients

The presented study involved 32 male subjects: 16 patients with ankylosing spondylitis (experimental group, mean age 47 ± 4.7 years) and 16 healthy volunteers (control group, mean age 43 ± 3.9 years). The difference in the age between both groups was not significant (p = 0.802). All patients included to the trial fulfilled the modified New York Criteria for definite diagnosis of AS, which serve as the basis for the ASAS/EULAR recommendations [17].

According to modified New York Criteria (1984) the

Results

The obtained results are shown in Table 1, Table 2, Table 3.

Plasma activity of total SOD and its isoenzyme SOD-ZnCu in patients with AS (13.78 ± 3.64 and 3.62 ± 3.00 NU/mL, respectively) were significantly lower in comparison with the control group (19.87 ± 3.07 and 9.10 ± 2.74 NU/mL, respectively).

In the lysates of erythrocytes of patients with AS a significantly lower activity of SOD (115.9 ± 68.37 vs. 218.9 ± 54.79 NU/mgHb), along with GPx (27.52 ± 14.55 vs. 47.64 ± 9.26 IU/gHb), GST (1.32 ± 1.89 vs. 2.85 ± 

Discussion

In numerous reports it has been proven that reactive oxygen species (ROS) play an essential role in the pathogenesis of inflammatory and degenerative diseases of the osteo-muscular system [29], [30], [31], [32], [33], [34], [35].

In the inflammatory disease of the locomotor system, ROS cause, among other things, peroxidation of cell membranes, depolymerization of the hyaluronic acid, degradation of proteoglycanes and collagen, oxidation of proteins, and inhibition of proliferation along with

Conclusion

The results of the study indicate that decrease in the antioxidant status leading to generation of oxidative stress may play an important role in the pathogenesis of ankylosing spondylitis.

Acknowledgments

This work was supported by grant of the State Committee for Scientific Research, Republic of Poland No 3 P05B 156 25.

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